The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products were being digested and cloned into a pET21a+ vector using NdeI and XhoI restriction enzymes. A degree mutation was introduced by the short transform https://barrettw221oam6.wikicorrespondence.com/user